Fig. 6

KRT6A inhibits the interaction between RNF41 and JAK1. A The Venn diagram of proteins interacted with JAK1 detected by IP-MS after transferring of Flag-JAK1 plasmid with or without KRT6A plasmid into HEK293T cells. B Flag-JAK1 and HA-RNF41 plasmids were transfected into HEK293T cells with or without KRT6A plasmid and the interaction between JAK1 and RNF41 was detected. C The JAK1 expression was detected in RNF41-overexpressed and enzymatic-null RNF41-expressed HEK293T cells. D The ubiquitination of JAK1 with or without RNF41 overexpression. E Schematic representation of RNF41 and its truncated forms. F Flag-JAK1, full-length HA-RNF41, or truncated mutants of RNF41 were coexpressed in HEK293T cells for co-IP assay with anti-Flag beads. G Flag-KRT6A and HA-JAK1 plasmids were transfected into HEK293T cells with or without RNF41 plasmid for co-IP assay with anti-Flag beads. All experiments were performed in three independent biological replicates