Fig. 5

KRT6A increases JAK1 expression by targeting the ubiquitination. The heat map (A), volcano graph (B) and GO analysis (C) of differentially expressed protein in KRT6A-knockdown HaCaT cells. D Representative IHC images showing the expression of JAK1 in rosacea lesions and healthy controls in the upper panel; Representative IHC images showing the expression of JAK1 in LL37-induced rosacea-like mice in the bottom panel. E The JAK1 expression in KRT6A-knockdown HaCaT cells. Quantification of relative protein expression is shown in the bottom panel. F The STAT3 activation in KRT6A-knockdown HaCaT cells combined with or without JAK1 overexpression. G The STAT3 activation in KRT6A-overexpressed HaCaT cells combined with or without JAK1 knockdown. H The mRNA levels of downstream cytokine in KRT6A-knockdown HaCaT cells combined with or without JAK1 overexpression. I The JAK1 expression in KRT6A-knockdown HaCaT cells treated with or without MG132. Quantification of relative protein expression is shown in the bottom panel. J The JAK1 expression treated with CHX in control or KRT6A-knockdown HaCaT cells. Quantification of relative protein expression is shown in the right panel. K The JAK1 expression treated with CHX in control or KRT6A-overexpressed HaCaT cells. Quantification of relative protein expression is shown in the right panel. L The ubiquitination of JAK1 with or without KRT6A overexpression. All experiments were performed in 3 independent biological replicates. Data represents the mean ± SEM. *P < 0.05; **P < 0.01, ***P < 0.001, ****P < 0.0001. Two-tailed unpaired Student’s t-test (E) or 1-way ANOVA with Bonferroni’s post hoc test (I, J, K) was used